Liquid biopsy and cell-free DNA

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For decades, histologic and pathologic analysis in routine oncology has been predominantly performed on tumor tissue obtained from biopsy or resection. However, this approach is invasive and does not come without risk, oftentimes not even being feasible due to advanced disease. Here, we introduce circulating cell-free DNA (cfDNA), which contains circulating-tumor DNA (ctDNA) in patients with cancer for precision oncology applications.

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a year ago by Samantha Hasenleithner & Michael Speicher — 12 min read

Understanding and estimating tumor fraction from plasma cell-free DNA

Vessel Tutorial - Understanding liquid biopsy results is highly dependent on tumor fraction of the cell-free DNA sample. Without information about ctDNA content from plasma, variant allele frequencies and uninformative testing results are very difficult to interpret.

concept Liquid biopsy and cell-free DNA